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目的:探讨MMP-2、MMP-9、TIMP-2、TIMP-1在糖尿病心肌病心室重构过程中的作用,以及糖心宁的心肌保护作用及机制。方法:36只SD大鼠,其中9只作为空白对照组,27只腹腔注射链脲佐菌素(STZ)制备糖尿病心肌病大鼠模型,随机分为模型组、糖心宁高剂量组、糖心宁等效剂量组3组,糖心宁组灌服中药,模型组及空白对照组灌服等量清洁饮用水,给药8周后处死大鼠,计算左心室肥厚指数,检测血清T-SOD活性和MDA含量;Masson染色观察心肌组织中胶原,免疫组化法检测心肌组织MMP-2、MMP-9、TIMP-2、TIMP-1的蛋白表达。结果:糖尿病心肌病大鼠用药8周后,糖心宁高剂量组、糖心宁等效剂量组均能降低左心室肥厚指数,减少MDA含量,升高T-SOD水平,且同时升高MMP-9/TIMP-1和MMP-2/TIMP-2比值,降低心肌胶原含量。结论:糖心宁具有通过改善氧化应激,重新调整MMP-9/TIMP-1平衡、减弱TIMP-1对MMP-9抑制从而减轻DCM心室重构的作用。
AIM: To investigate the role of MMP-2, MMP-9, TIMP-2 and TIMP-1 in ventricular remodeling in diabetic cardiomyopathy and its protective mechanism and mechanism. Methods: Thirty-six SD rats, of which 9 were used as blank control group. 27 diabetic rats were injected intraperitoneally with streptozotocin (STZ), and were randomly divided into model group, high-dose sugar syrup group, Xinning equivalent dose group of three groups, sugar Xin Ning group were fed with traditional Chinese medicine, model group and blank control group were fed equal amounts of clean drinking water, 8 weeks after administration of rats were sacrificed to calculate the left ventricular hypertrophy index, serum T- The activity of SOD and the content of MDA in myocardium were detected by Masson staining. The protein expression of MMP-2, MMP-9, TIMP-2 and TIMP-1 in myocardium was detected by immunohistochemistry. Results: After 8 weeks of treatment, rats in diabetic cardiomyopathy group were significantly lower than those in the high-dose syngas and syngangning groups for reducing LVH, decreasing MDA content, increasing T-SOD level and increasing MMP -9 / TIMP-1 and MMP-2 / TIMP-2 ratio, reduce myocardial collagen content. Conclusion: Tangxinning has the effect of decreasing oxidative stress, re-adjusting the balance of MMP-9 / TIMP-1 and attenuating the inhibition of MMP-9 by TIMP-1.