采用二阶导数光谱法测定溴新斯的明磷脂复合物的复合率,以264.2nm处测定振幅D值,测定溴新斯的明磷脂复合物中溴新斯的明的含量,由此计算复合率。结果表明,溴新斯的明在0.10—0.40mg·mL-1浓度范围内线性关系良好,回归方程为y=0.3057x+0.0053,r=0.9992;平均加样回收率为99.86%,RSD为1.16%(n=9)。此方法准确,简便,快速,适用于溴新斯的明磷脂复合物复合率的测定。 The second derivative spectrophotometry was used to determine the recombination rate of bromophos-nes phospholipid complex. The amplitude of bromodiazepine was determined at 264.2 nm. rate. The results showed that there was a good linear relationship between bromine and neostigmine in the concentration range of 0.10-0.40 mg · mL-1, the regression equation was y = 0.3057x + 0.0053, r = 0.9992; the average recovery was 99.86% with a RSD of 1.16 % (n = 9). The method is accurate, simple and rapid, and is suitable for the determination of complex ratio of phospholipid complex of bromine neos.