龙胆花是日本东北地区盛产的一种花卉，在我国也有栽培。此花采用种子繁殖，而种子繁殖往往存在品种退化问题。本试验采用叶片为外植体，进行离体大量繁殖，目的是为保持龙胆花的品种特性提供一条生产途径。１　材料与方法　供试品种为ＷＳＰ－３。作为外植体的叶片直接取自试 Gentian flower is a flower rich in northeastern Japan, also cultivated in our country. This flower uses seeds to breed, and seed breeding often has the problem of variety degeneration. In this experiment, leaves were used as explants for large-scale reproduction in vitro. The purpose of this experiment is to provide a production route to maintain the variety characteristics of gentian flowers. 1 Materials and methods The test varieties WSP-3. Leaves as explants were taken directly from the test