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克隆了棉铃虫Helicoverpaarmigera单粒包埋型核型多角体病毒 (HaSNPV)C1株基因组DNA ,并通过随机测序的方法测定了经XbaI酶切后的H片段的核苷酸全序列。序列比较和分析发现该片段中ORF1 3与苜蓿丫纹夜蛾Autographacalifornica多粒包埋型核型多角体病毒 (AcMNPV)基因组ORF1 47(ie 1 )同源。ie 1基因编码区全长 1 986bp ,根据推测的氨基酸序列 ,可编码 6 6 1个氨基酸残基组成的多肽 ,预计分子量为 76 .5kD。将所推导的HaSNPVIE 1氨基酸序列与其它已知的杆状病毒IE 1氨基酸序列进行比较 ,结果表明 ,HaSNPV和谷实夜蛾H .zea单粒包埋型核型多角体病毒IE 1氨基酸序列最为相似 ,同源性高达 98%。与AcMNPV、家蚕Bombyxmori核型多角体病毒 (BmNPV)、云杉卷叶蛾Choristoneurafu miferana多粒包埋型核型多角体病毒 (CfMNPV)、舞毒蛾Lymantriadispar多粒包埋型核型多角体病毒(LdMNPV)、黄杉毒蛾Orgyiapseudotsugata多粒包埋型核型多角体病毒 (OpMNPV)、甜菜夜蛾Spodopteraex igua多粒包埋型核型多角体病毒 (SeMNPV)、小菜蛾Plutellaxylostella颗粒体病毒 (PxGV)和Xestiac ni grum颗粒体病毒 (XcGV)的IE 1氨基酸序列同源性较低 ,分别为 2 3 %、2 3 %、2 3 %、2 5 %、2 3 %、1 4%、2 7%和 7%。根据氨基酸序列由GENETYX
The genomic DNA of Helicoverpa armigera single-nucleopolyhedrovirus (HaSNPV) C1 strain was cloned, and the complete nucleotide sequence of H fragment digested with XbaI was determined by random sequencing. Sequence comparison and analysis revealed that ORF1 3 was homologous with the ORF1 47 (ie 1) of the Autographa californica multi-nucleopolyhedrovirus (AcMNPV) genome. ie 1 gene coding region of a total length of 1 986bp, based on the deduced amino acid sequence, can encode a polypeptide consisting of 6 6 1 amino acid residues, the expected molecular weight of 76.5kD. The deduced amino acid sequence of HaSNPVIE 1 was compared with other known baculovirus IE 1 amino acid sequences. The results showed that the amino acid sequence of IE 1 of HaSNPV and Helicoverpa sanguinea H. Zea single-particle-embedded nuclear polyhedrosis virus was the most Similar, homology up to 98%. And AcMNPV, Bombyx mori nuclear polyhedrosis virus (BmNPV), Choristoneurafu miferana multifractory nuclear polyhedrosis virus (CfMNPV), Lymantriadispar multidrug-embedded nuclear polyhedrosis virus (LdMNPV) (OpMNPV), Spodoptera exigua multi-nucleopolyhedrovirus (SeMNPV), Plutella xylostella granulosis virus (PxGV) and Xestiac ni grum The IE1 amino acid sequence homology of the granzyme virus (XcGV) was 23%, 23%, 23%, 25%, 23%, 14%, 27% and 7%, respectively. According to the amino acid sequence by GENETYX