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目的探讨神经生长因子(NGF)在哮喘发病中对气道炎症TNF-αmRNA表达的调节作用。方法实验分为对照组、哮喘组、NGF阻断组。免疫组化方法结合显微图像分析检测NGF的表达,以及抗NGF干预后哮喘的发作情况、嗜酸性粒细胞及TNF-αmRNA表达的关系。结果 NGF阻断组与哮喘组比较,哮喘发作次数、嗜酸性粒细胞计数显著减少(均P<0.01),肺组织炎症表现显著减轻。哮喘组大鼠肺组织内NGF mR-NA表达水平较对照组显著增高(P<0.05),NGF阻断组肺组织内TNF蛋白及TNF-αmRNA的表达较哮喘组明显减少(P<0.05)。结论 NGF阻断可减少肺部嗜酸粒细胞浸润、哮喘的发作次数及TNF-αmRNA蛋白的表达,并可以抑制气道炎症。
Objective To investigate the regulatory effect of nerve growth factor (NGF) on the expression of TNF-αmRNA in airway inflammation in the pathogenesis of asthma. Methods The experiment was divided into control group, asthma group and NGF blocking group. Immunohistochemistry and microscopic image analysis were used to detect the expression of NGF and the relationship between asthma attack and eosinophils and TNF-αmRNA expression after anti-NGF intervention. Results Compared with asthma group, the numbers of asthma attack and eosinophil count in NGF block group were significantly decreased (all P <0.01), and lung inflammation was significantly reduced. Compared with the control group, the expression of NGF mR-NA in the lung tissue of asthmatic rats was significantly increased (P <0.05). The expression of TNF-α and TNF-αmRNA in the lung tissue of NGF-treated rats was significantly decreased compared with the asthma group (P <0.05). Conclusion NGF blockade can reduce pulmonary eosinophil infiltration, the number of asthma attacks and TNF-αmRNA protein expression, and can inhibit airway inflammation.