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目的研究德格县喜马拉雅旱獭鼠疫自然疫源地情况。方法对2008年在德格县捕获的喜马拉雅旱獭等材料进行细菌分离培养、胶体金免疫层析法(GICA)与鼠疫间接血凝(IHA)和反向血凝(RIHA)。结果细菌培养检测自毙喜马拉雅旱獭26份,分离鼠疫菌9株;IHA检测旱獭血清79份、阳性3份,牧犬血清29份、阳性3份;RIHA检测组织悬液30份、阳性9份均为旱獭;GICA检测旱獭血清79份、阳性2份,牧犬血清29份、阳性9份,藏系绵羊血清32份、阳性8份;GICA抗原检测组织悬液30份,阳性11份(旱獭10份、家猫1份)。结论从德格县喜马拉雅旱獭分离出鼠疫菌,说明四川省德格县2008年正在发生动物鼠疫流行。
Objective To study the natural epidemic situation of plague of Marmota marmota in Dege County. Methods Bacterial isolates were collected from Himalayan marmot captured in Dege County in 2008. GICA and IHA and RIHA were also isolated. Results Bacterial culture was detected in 26 Himalayan Marmots, 9 strains were isolated from Yersinia pestis. 79 strains of Marmota serrata were detected by IHA, 3 were positive and 29 were seropositive, and 3 were positive. RIHA detected 30% For the marmot; GICA detection sericin 79, positive 2, dog serum 29, 9 positive, Tibetan sheep serum 32, positive 8; GICA antigen test tissue suspension 30, positive 11 (Marmot 10 Copies, 1 cats). Conclusion The isolation of Yersinia pestis from Himalayan marmot in Dege County shows that the outbreak of plague in 2008 is occurring in Dege County, Sichuan Province.