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钆离子的配合物作为核磁共振造影增强剂在临床诊断中得到广泛应用。晚期肾病患者多次大剂量使用钆造影剂后,可能发生肾源性系统纤维化,目前其确切机理还不清楚。我们考察了不同浓度的氯化钆对人脐静脉内皮细胞的迁移和成管能力的影响;在鸡胚尿囊膜模型中,10μM氯化钆诱导生成更多的新生血管。这些实验结果表明氯化钆具有促进人脐静脉内皮细胞体外血管形成能力,这种作用涉及细胞内活性氧物种和钙离子水平的变化,以及PKCα/β2和MAPKs信号通路的激活。该研究为探索钆造影剂和肾源性系统纤维化之间的关系提供了新线索。
Gadolinium complexes as MRI enhancer in clinical diagnosis has been widely used. Nephrotic systemic fibrosis may occur after multiple doses of gadolinium contrast agent in patients with advanced renal disease, and its exact mechanism is unclear. We examined the effect of different concentrations of gadolinium chloride on the migration and tube formation of human umbilical vein endothelial cells; in the chick chorioallantoic membrane model, 10 μM gadolinium chloride induced more angiogenesis. These experimental results indicate that gadolinium chloride has the ability to promote the in vitro angiogenesis of human umbilical vein endothelial cells, which is involved in the changes of intracellular reactive oxygen species and calcium levels and the activation of PKCα / β2 and MAPKs signaling pathways. This study provides new clues to explore the relationship between gadolinium contrast agents and nephrogenic fibrosis.