目的　研究脑活素 (现药名施普善 )对原代培养的大鼠小脑颗粒神经元的影响。方法　采用原代培养的大鼠小脑颗粒神经元 ,观察脑活素对细胞形态学及生存的影响。用噻唑蓝 (MTT)法测定细胞存活率。结果　脑活素在 0～ 40mL/L浓度下 ,剂量依赖性诱导小脑颗粒神经元死亡。兴奋性氨基酸受体拮抗剂地佐环平 (MK -80 1)可保护低浓度脑活素 (<5 0mL/L)的神经毒性。脑活素浓度 >5 0mL/L时MK - 80 1无明显保护作用。而对照药物胞二磷胆碱 (0～ 10g/L)、单唾液酸四己糖神经糖甙脂 (GM - 1,0～ 2 0mg/L)两者被认为具有护脑作用的药物 ,对小脑颗粒细胞均无毒性作用。结论　脑活素对原代培养的大鼠小脑颗粒神经元具有毒性作用 ,其机制部分是通过兴奋性氨基酸介导的。提示脑活素的神经药理作用值得进一步探讨 Objective To study the effects of cerebrolysin (now known as Shi Pu Shan) on primary cultured rat cerebellar granule neurons. Methods Primary cultured rat cerebellar granule neurons were used to observe the effect of cerebrolysin on cell morphology and survival. Cell viability was measured by MTT method. Results Cerebrolysin dose-dependently induced the death of cerebellar granule neurons at concentrations of 0 to 40 mL / L. Excitatory amino acid receptor antagonist, dizolozepine (MK-801), can protect neurotoxicity of low concentrations of cerebrolysin (<50 mL / L). There was no significant protective effect of MK - 80 1 at the concentration of> 50 mL / L. The control drugs citicoline (0 ~ 10g / L), monosialotetrahexosylcelloglycosides (GM - 1, 0 ~ 20mg / L) are considered to have a brain-protective effect of the drug, Cerebellar granulosa cells have no toxic effect. Conclusion Cerebrolysin has toxic effects on primary cultured rat cerebellar granule neurons, and its mechanism is partly mediated by excitatory amino acids. Suggest that the neuropharmacological role of cerebrolysin warrants further investigation