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研究以上皮型胸膜间皮瘤,纤维型胸膜间皮瘤,结核型胸膜炎与正常胸膜组织为材料,通过傅里叶变换红外光谱分析组织中生物大分子的结构及含量的改变。研究发现,四种胸膜组织的傅里叶红外光谱较为相似,但存在明显区别,四种胸膜组织红外光谱数据方差差异极显著(sig.<0.001),说明胸膜间皮瘤中生物大分子的含量及结构发生了明显变化,主要包括:(1)胸膜间皮瘤蛋白质酰胺Ⅰ带及酰胺Ⅱ带,核酸1 232cm-1峰强,脂类物质2 922cm-1峰强均显著高于正常人胸膜组织,与正常胸膜组织存在显著区别;纤维型胸膜间皮瘤中,蛋白质酰胺Ⅰ带及酰胺Ⅱ带峰强,与核酸密切相关的1 078cm-1峰强,以及与脂类物质相关的2 922和2 854cm-1峰强均显著高于上皮型胸膜间皮瘤(p<0.05);结核型胸膜炎蛋白质酰胺Ⅰ带及酰胺Ⅱ、核酸1 232和1 078cm-1峰强略有增加,但与正常胸膜组织差异不显著(p>0.05),与脂类物质含量有关2 922cm-1峰强、2 854cm-1峰强,极显著地高于正常胸膜组织(p<0.01),显著高于胸膜间皮瘤(p<0.05)。(2)蛋白质、核酸、脂类物质的相对峰强I1 641/I2 922,I1 641/I1 232,I1 232/I1 078,I1 078/I1 546,I1 078/I2 854,I2 922/I1 232,I1 458/I1 400能有效放大四类胸膜组织间的差异,其效果优于峰强效果,可作为胸膜间皮瘤诊断的优化指标。(3)上皮型胸膜间皮瘤中指示核酸分子中磷酸二酯键的C—C/C—O的1 078cm-1峰强以及指示脂类物质的2 854cm-1峰强显著低于纤维型胸膜间皮瘤和正常胸膜组织(p<0.05),表明上皮型胸膜间皮瘤中磷酸二酯键断裂程度较高,DNA受损严重,膜脂过氧化降解明显。说明上皮型胸膜间皮瘤恶化程度高于纤维型胸膜间皮瘤。(4)胸膜间皮瘤蛋白质酰胺Ⅰ带、Ⅱ带谱带、核酸1 232cm-1峰、脂类物质1 458cm-1处CH2振动及1 400cm-1处CH3振动红移,说明蛋白质分子间的氢键受到破坏,核酸分子的氢键结合力减弱,核酸分子的双链结构受到一定程度的破坏,肿瘤组织中膜脂的亚甲基链趋向无序。(5)傅里叶红外光谱能有效区分纤维型胸膜间皮瘤、上皮型胸膜间皮瘤、结核型胸膜炎、正常胸膜组织,为胸膜间皮瘤与结核型胸膜炎的早期、快速诊断提供了可靠的数据。
To study the changes of the structure and content of biological macromolecules in tissues with epithelial pleural mesothelioma, fibrous pleural mesothelioma, tuberculous pleurisy and normal pleural tissue as materials. The results showed that the FTIR spectra of the four pleural tissues were similar, but there was a significant difference between the four pleural tissue FTIR data (sig. <0.001), indicating that the content of biological macromolecules in pleural mesothelioma (1) Pleural mesothelioma protein amide Ⅰ band and amide Ⅱ band, 1 232cm-1 nucleic acid peak intensity, lipid peak intensity of 2 922cm-1 were significantly higher than normal human pleura There was a significant difference between normal pleural tissue and fibrous pleural mesothelioma. The peak area of protein amide Ⅰ and amide Ⅱ was strongly associated with nucleic acid, and the peak intensity of 1 078cm-1, which was related to lipids, And 2 854cm-1 peak intensity were significantly higher than epithelial pleural mesothelioma (p <0.05); tuberculous pleurisy protein amide Ⅰ band and amide Ⅱ, 1 232 and 1 078cm-1 peak intensity slightly increased, but with The normal pleural tissue was not significantly different (p> 0.05), and peaked at 2922cm-1 and 2 854cm-1, which was significantly higher than that of normal pleural tissue (p <0.01), which was significantly higher than that of pleura Mesothelioma (p <0.05). (2) Relative peak intensity of proteins, nucleic acids and lipids I1 641 / I2 922, I1 641 / I1 232, I1 232 / I1 078, I1 078 / I1 546, I1 078 / I2 854, I2 922 / I1 232, I1 458 / I1 400 can effectively amplify the difference between the four types of pleural tissue, the effect is better than the peak intensity effect, which can be used as an optimal index for the diagnosis of pleural mesothelioma. (3) The peak intensity of 1 078 cm-1 of C-C / C-O indicating phosphodiester bond in the nucleic acid molecule and the peak intensity of 2 854 cm-1 indicating lipid in the epithelial pleural mesothelioma were significantly lower than those of the fibro- Pleural mesothelioma and normal pleural tissue (p <0.05), indicating that the epithelial type of pleural mesothelioma phosphodiester bond higher degree of damage, DNA damage, membrane lipid peroxidation significantly. Description of epithelial pleural mesothelioma worse than fibrosis pleural mesothelioma. (4) Pleural mesothelioma protein amide Ⅰ band, Ⅱ band, 1 232cm-1 peak of nucleic acid, lipid substance 1 458cm-1 CH2 vibration and 1 400cm-1 CH3 vibration redshift, indicating the intermolecular protein The hydrogen bond is damaged, the hydrogen bonding ability of the nucleic acid molecules is weakened, the double-stranded structure of the nucleic acid molecule is damaged to some extent, and the methylene chain of the membrane lipid in the tumor tissue tends to be disorderly. (5) Fourier transform infrared spectroscopy can effectively distinguish fibrous pleural mesothelioma, epithelial pleural mesothelioma, tuberculous pleurisy, normal pleural tissue, pleural mesothelioma and tuberculous pleurisy early, rapid diagnosis provides a reliable The data.