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Objective:To identify potential serum markers of hepatic carcinoma in rats through Surface-Enhanced Laser Desorption Ioniza- tion-Time of Flight-Mass Spectrometry(SELDI-TOF-MS)Technology.Methods:A rat model of hepatic carcinoma was established. The serum samples of hepatic carcinoma and normal rats were analyzed via SELDI-TOF-MS Technology.The changes of the serum protein fingerprint patterns were observed between the experimental group of hepatic carcinoma and the controls.The analysis was conducted by statistical software-Biomarker Wizard.Results:Fifty-six protein peaks in the serums were found.Within m/z 0-20 000, the protein peaks of m/z 1158,8 835 and 15 302 of hepatic carcinoma serums were obviously higher in the rat models compared with those in the controls(P<0.01).Conclusion:Three peaks were considered as potential biomarkers according to the serum protein fingerprint patterns of the hepatic carcinoma group and the control group.
Objective: To identify potential serum markers of hepatic carcinoma in rats through Surface-Enhanced Laser Desorption Ioniza- tion-Time of Flight-Mass Spectrometry (SELDI-TOF-MS) Technology. Methods: A rat model of hepatic carcinoma was established. The serum samples of hepatic carcinoma and normal rats were analyzed via SELDI-TOF-MS Technology. changes of the serum protein fingerprint patterns were observed between the experimental group of hepatic carcinoma and the controls. analysis was conducted by statistical software-Biomarker Wizard. Results : Fifty-six protein peaks in the serums were found. Whithin m / z 0-20 000, the protein peaks of m / z 1158, 8 835 and 15 302 of hepatic carcinoma serums were obviously higher in the rat models compared with those in the controls (P <0.01) .Conclusion: Three peaks were considered as potential biomarkers according to the serum protein fingerprint patterns of the hepatic carcinoma group and the control group.