冬虫夏草菌丝体生产菌种C_(618)的特性研究

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冬虫夏草是一种名贵的药用菌.它与一般的食用药用菌(担子菌类)有明显的区别.该项目经4年时间的研究、分析、测定认为,从海拔2500m以上采集,并经分离、驯化、培养、测定的菌种C_(618)是1株有特性的冬虫夏草菌丝体生产菌.现将其主要特性报告如下.形态特征:①菌落在PDA培养基上,25℃培养4d,直径3.2cm,绒状,边缘整齐,分泌液透明.②菌丝体由弯曲的丝状组成,菌丝有分枝、分隔.③子囊壳座垫.具有数个侧生或丛生的子囊:子囊细、棒状、顶部尖锐,高约2~30mm.直径0.2~0.5mm.生理特征:①菌种纯正,独特,经固体、液体培养,菌丝在前期生长洁白.后期有少数红色色素产生.②寄生虫体专一,为鳞翅目的幼虫,似四眼老蚕,长3~5cm,外表淡黄至黄棕色,腹有足8对,中部4对明显.③培养周期短,斜面培养8d,三角瓶培养5d,发酵罐培养84h(3.5d).④生产产量高,一般生产菌生产的菌丝体为1000mL培养基,得干燥纯菌丝体6-8g.利用C_(618)菌种.1000mL培养基得干燥纯菌丝体8—9g(注:相同条件下).⑤产品质量好,药用价值高,用C_(618)菌种生产的菌丝体经陕西中医研究所、陕西产品质量检验所等单位分析测定,药用成分均高于或等于天然冬虫夏草.经陕西省保健品检测中心、陕西省卫生防疫站等单位检测,产品无毒,无副作用,卫生指标等符合国家标准.产品经? Cordyceps sinensis is a precious medicinal fungus.Its general medicinal fungi (basidiomycetes) there is a clear distinction.The project after 4 years of research, analysis, determination that, from more than 2500m above sea level and by The strain C_ (618), which was isolated, domesticated and cultured, was identified as one of the producing strains of Cordyceps sinensis, and its main characteristics were reported as follows: Morphological characteristics: ① The colonies were cultured on PDA medium at 25 ℃ for 4 days , Diameter 3.2cm, velvet, neat edge, exudate transparent .② mycelium consists of curved filaments, mycelium branches, separated. ③ Oocysts seat cushion with a few lateral or clustered ascus: Subcapsular thin, rod-shaped, top sharp, about 2 ~ 30mm. Diameter 0.2 ~ 0.5mm. Physiological characteristics: ① Strains pure, unique, solid, liquid culture, mycelium grew white in the early stage of a small number of red pigment. ② parasitic body-specific, for Lepidoptera larvae, like four old silkworms, long 3 ~ 5cm, the appearance of yellow to yellow-brown, abdomen with 8 pairs of feet, the central 4 pairs of obvious ③ training cycle is short, beveling 8d , Flask culture 5d, fermenter culture 84h (3.5d) .④ high production, the general production of mycelium production of 1000m L medium, dried pure mycelium 6-8g using C_ (618) strains. 1000mL medium was dried pure mycelium 8-9g (Note: under the same conditions.) ⑤ product quality, medicinal value High, with C_ (618) strains of mycelium produced by the Shaanxi Institute of Traditional Chinese Medicine, Shaanxi product quality inspection and other units analyzed, the medicinal ingredients are higher than or equal to natural Cordyceps sinensis by Shaanxi Health Products Testing Center, Shaanxi Provincial Health and Epidemic Prevention Station and other units testing, non-toxic products, no side effects, health indicators in line with national standards.
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