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用全薯块吸头刺伤接种法测定时,较理想的测定条件为:每接种点接种0.1ml浓度为10~2～10~5CFU/ml菌悬液,随后在21±5℃～31±5℃(依所用菌株而定)下保持2～3天,以接种点腐烂斑的最大直径作记载标准。用新鲜薯片法测定时,除接种浓度为10~2～10~3CFU/ml,保持时间为1～2天及以侵染限值作评价标准外,其余条件和全薯块吸头刺伤接种法相同。当用皮孔浸渍法接种时,薯块在10_6CFU/ml菌悬液中浸5分钟,然后使薯块表面保持连续水膜,3～5天后测量薯块表面腐烂面积。上述3种方法都必须有20个以上重复,并使用大小一致、无伤无病的成熟薯块。
When the inoculation method is used to determine the whole potato tuber-piercing stab inoculation, the ideal measuring conditions are as follows: 0.1 ml of bacterial suspension is inoculated at a concentration of 10 ~ 2 ~ 10 ~ 5CFU / ml for each inoculation point and then inoculated at 21 ± 5 ° C ~ 31 ± 5 ℃ (depending on the strains used) for 2 to 3 days, the maximum diameter of the point of seeding rot for recording standards. When measured by the method of fresh potato chips, in addition to inoculation concentration of 10 ~ 2 ~ 10 ~ 3CFU / ml, holding time of 1 ~ 2 days and the infection limit for the evaluation criteria, Inoculation method the same. When inoculated with leachate, the tubers were soaked in 10-6 CFU / ml bacterial suspension for 5 minutes, and then the water surface of the tubers was kept in a continuous water film. The rotten area of the tubers was measured after 3 to 5 days. The three methods must have more than 20 repetitions, and use the same size, non-injury disease-free mature potato.