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探讨鳖甲多肽的纯化、鉴定及其体外抗肝纤维化活性.通过超滤、葡聚糖凝胶Sephadex G-15及高效液相色谱法对鳖甲水解产物进行分离,得到一个纯化的寡肽(CTEPH-1∶Asn-Pro-Asn-Pro-Thr).采用MTS法和酶联免疫吸附法测定纯化多肽对活化肝星状细胞的抑制作用,发现通过降低各型胶原蛋白和TIMP-1的含量,CTEPH-1有效的抑制了肝星状细胞的活化和增殖.因此,该寡肽CTEPH-1可以作为功能食品的添加剂或潜在的抗肝纤维化药物.“,”Anti-hepatic fibrosis peptide from Carapax Trionycis was purified,characterized,and inhibitory effect was assessed.Carapax Trionycis extract peptide hydrolysates (CTEPHs) were separated by ultrafiltration,Sephadex G-15 gel chromatography and RP-HPLC.One novel anti-hepatic fibrosis peptide (CTEPH-1:Asn-Pro-Asn-Pro-Thr) was obtained and identified.MTS assay and enzyme-linked immunosorbent assay were applied to evaluate the anti-fibrotic effect of CTEPH-1 on activated hepatic stellate cells (HSCs) in vitro.CTEPH-1 efficiently inhibited activation and proliferation of cultured HSC-T6 cells via lowering the contents of collagen and TIMP-1 except for matrix metalloproteinase-1 (MMP-1).The purified peptide might be beneficial as functional food or potential drug for treatment of liver fibrogenesis.