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在通常的酶免疫分析中,辣根过氧化物酶(HR-P)与抗体 IgG 以共价联接,但往往使 HRP 结合能力丧失50%,而且联接物中 HRP 的活性也会降低75%。为此,本文作者采用 HRP 与抗 HRP 抗体,通过免疫联接,形成一种可溶性的 PAP 复合物,从而克服 HRP 失活的缺点,并做为一种通用的试剂,应用于简化酶免疫测定(EIA)和 PAPEIA 测定。简化 EIA 的原理是 PAP 复合物直接与第二抗体包被固相进行免疫结合;而 PAP-EIA 的原理是:
In a typical enzyme immunoassay, horseradish peroxidase (HR-P) is covalently linked to an antibody IgG, but often results in a 50% loss of HRP binding capacity and a 75% decrease in HRP activity in the linker. To this end, the authors used HRP and anti-HRP antibodies to form a soluble PAP complex by immunosuppression to overcome the disadvantages of HRP inactivation and as a general-purpose reagent for the simplified enzyme immunoassay (EIA ) And PAPEIA assay. The principle of Simplifying EIA is that the PAP complex is directly immunosorbed onto the second antibody coated solid phase. The principle of PAP-EIA is that: