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目的 :揭示正常胃粘膜、癌前病变和胃癌组织中MUC5AC基因的表达与其临床病理分型之间的联系。方法 :应用免疫组织化学SP法检测人正常胃粘膜、癌前病变粘膜以及胃癌组织中MUC5AC核粘蛋白的表达。结果 :人正常胃粘膜中的浅表 1/ 3范围内广泛分布MUC5AC基因产物 ( 10 0 % ) ,肠化、不典型增生和胃癌组织中的表达阳性率分别是 2 9.6%、10 0 %和 4 0 .0 %。胃癌组织中MUC5AC核粘蛋白的表达与胃癌患者的性别、肿瘤的部位和大小、淋巴结转移、肿瘤的分化和浸润、临床分期无关 (P >0 .0 5 ) ,但MUC5AC阳性组与阴性组之间的胃癌的Lau ren’氏分型差别显著 (P <0 .0 5 )。结论 :MUC5AC是组成胃粘膜的粘蛋白基因之一 ,在胃癌和癌前病变是下调表达 ,MUC5AC核粘蛋白与胃癌的Lauren’氏分型有关
Objective: To reveal the relationship between MUC5AC gene expression and clinicopathological types in normal gastric mucosa, precancerous lesions, and gastric cancer tissues. METHODS: Immunohistochemical SP method was used to detect the expression of MUC5AC nuclear mucin in normal gastric mucosa, precancerous mucosa and gastric cancer. RESULTS: MUC5AC gene products (100%) were widely distributed in the superficial 1/3 of normal human gastric mucosa, and the positive rates in intestinal metaplasia, dysplasia, and gastric cancer tissues were 29.6%, 100%, respectively. 4 0 .0 %. The expression of MUC5AC nuclear mucin in gastric cancer tissues was not associated with gender, location and size of tumor, lymph node metastasis, tumor differentiation and invasion, and clinical stage (P > 0.05), but MUC5AC positive group and negative group. There was a significant difference in the Lau ren’s genotype between gastric cancers (P < 0.05). Conclusion : MUC5AC is one of the mucin genes that make up the gastric mucosa. It is down-regulated in gastric cancer and precancerous lesions. MUC5AC nuclear mucin is related to Lauren’s classification of gastric cancer.