论文部分内容阅读
采用反相高效液相色谱法，选择苯酚为内标物，ＺＹ１１０４型ＸＷＧ－Ｃ１８柱（４．６ｍｍ×２５０ｍｍ）为分析柱，甲醇－水－０．２ｍｏｌ／Ｌ硫酸铵（１０．６８０．７２，ｐＨ＝７．７）为流动相，流速为１．０ｍＬ／ｍｉｎ，检测波长为２５４ｎｍ，进样量２０μＬ，测定片剂中盐酸莫索尼定的含量，按盐酸莫索尼定计算，理论板数为２５４４，样品峰与内标峰的分离度为３．９．线性范围为４～１００ｍｇ／Ｌ（ｒ＝０．９９９８），最低检测量为１０ｎｇ，平均回收率为９９．８２％，ＲＳＤ＝０．７９％．用该法测定３批盐酸莫索尼定片剂的含量，结果分别为（９６．８±１．９）％、（９９．４±１．７）％和（９９．３±１．６）％．本法快速、灵敏、准确，专属性高．
Using reversed-phase high performance liquid chromatography, phenol was selected as internal standard, ZY1104 XWG-C18 column (4.6mm × 250mm) as analytical column, methanol-water -0.2mol / L ammonium sulfate (10.680.72 , PH = 7.7) as the mobile phase, the flow rate of 1.0mL / min, the detection wavelength of 254nm, the injection volume of 20μL, determination of the content of moxonidine hydrochloride tablets, Is 2544, the separation of the sample peak from the internal standard peak is 3.9. The linear range was 4 ~ 100 mg / L (r = 0.9998). The lowest detection limit was 10 ng. The average recovery was 99.82% and RSD was 0.79%. The contents of three batches of moxonidine hydrochloride tablets were (96.8 ± 1.9)%, (99.4 ± 1.7)% and (99.3 ± 1.6)%, respectively . This law is fast, sensitive, accurate and highly specific.