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目的构建慢性社会应激模型,探究慢性社会应激对雄性C57BL/6小鼠生殖健康的影响。方法将C57BL/6小鼠分成应激组和对照组;应激组予以慢性社会应激25d后,通过社会交互作用的行为学实验和体质量变化评估应激模型的构建状况,并对比两组小鼠生殖腺质量、精子计数等指标评估生殖健康的影响。结果慢性社会应激结束后,通过社会交互作用的行为学实验,发现应激组小鼠在行为上具有明显社会逃避行为;同对照组相比应激组小鼠生殖腺质量、精子数目均明显降低。结论慢性社会应激可以损伤雄鼠生殖功能,主要表现在生殖腺萎缩、精子数目减少等。
Objective To establish a chronic social stress model to explore the impact of chronic social stress on reproductive health of male C57BL / 6 mice. Methods C57BL / 6 mice were divided into stress group and control group. After 25 days of chronic social stress in stress group, behavioral tests and body mass changes of social interaction were used to evaluate the construction of stress model. Mouse gonadal quality, sperm count and other indicators to assess the impact of reproductive health. Results After the end of chronic social stress, behavioral experiments of social interaction found that stress-induced mice had obvious social evasion behavior. Compared with the control group, the gonad mass and the number of sperm in stress-induced mice decreased significantly . Conclusion Chronic social stress can damage male reproductive function, mainly in the gonadal atrophy and the reduction of the number of sperm.