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目的:研究向日葵列当的抗氧化活性。方法:采用DPPH.方法对向日葵列当双液相萃取中水相的石油醚、二氯甲烷、乙酸乙酯、饱和正丁醇4个组分进行抗氧化活性研究,采用硅胶、Sephadex LH-20等色谱技术对乙酸乙酯组分进行分离,并对所分离出的单体化合物进行了结构鉴定和抗氧化活性研究。结果:水相4个萃取组分中,饱和正丁醇组分对DPPH.自由基的清除能力最强,乙酸乙酯组分次之,石油醚和二氯甲烷组分较弱。经波谱分析及理化性质鉴定,从乙酸乙酯组分分离出的单体化合物的结构为1,2-O-[2S-(3,4-二羟基苯基)-l,2-乙烷二基]-3-O-α-L-鼠李吡喃糖基-4-O-咖啡酰基-β-D-葡萄吡喃糖苷。结论:此化合物首次从该植物中分得,具有较强的抗氧化活性。
Objective: To study the antioxidant activity of sunflower. Methods: The antioxidant activity of four fractions of petroleum ether, methylene chloride, ethyl acetate and saturated n-butanol in the aqueous phase extracted by two-phase extraction of sunflower was studied by DPPH method. Silica gel, Sephadex LH-20 The chromatographic techniques were used to separate the ethyl acetate components. The structures of the isolated compounds were identified and their antioxidative activities were studied. Results: Saturated n-butanol fraction showed the strongest DPPH radical scavenging activity in the four aqueous extracts, ethyl acetate followed by petroleum ether and methylene chloride. The structure of the monomer compound isolated from the ethyl acetate fraction was identified by spectral analysis and physico-chemical characterization as 1,2-O- [2S- (3,4-dihydroxyphenyl) -l, 2- Yl] -3-O-α-L-rhamnopyranosyl-4-O-caffeoyl-β-D-glucopyranoside. Conclusion: The compound was isolated from this plant for the first time and has strong antioxidant activity.