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Lister描述了苹果凹茎病毒(SGV)及这种病毒引起敏感的草本寄主的症状。这种病毒引起苹果疾病,经热处理后,病毒仍未被消灭。大多数苹果品种受SGV病毒感染,但并未显示出任何症状。由于一些品种具有这种敏成性,因此,州间和国际间对未获得无SGV病毒证明的果树进行检疫,不准予出售。因为Griffith品种全部无性系都受到SGV病毒的感染,所以我们就着手用茎尖培养来获得健康植株。配制了一种令人满意的培养基,但这种培养基不能诱导生根。培养基组成成分有MurashigeSkoog(MS)矿物盐、Gamborg等的各种维生素,再加上2%蔗糖、0.5μm6-苄基氨基嘌呤和0.1μm GA_3。我们已经成功地在试管内进行了苹果微型嫁接。这里我们研究了一些影响嫁接成功和测
Lister describes the apple concave virus (SGV) and the virus causes the symptoms of sensitive host herbs. This virus causes apple disease, the virus has not been eliminated after heat treatment. Most apple varieties are infected with the SGV virus but do not show any symptoms. Due to the sensitivity of some varieties, interstate and international quarantine of fruit trees that do not have SGV proof is not allowed. Because all Griffith clones are infected with the SGV virus, we set out to cultivate healthy plants with tips. A satisfactory medium was formulated but this medium failed to induce rooting. The medium consisted of Murashige Skoog (MS) mineral salts, various vitamins such as Gamborg, plus 2% sucrose, 0.5 μm 6-benzylaminopurine and 0.1 μm GA_3. We have successfully micro-grafted apple in a test tube. Here we study some of the effects of grafting success and measurement