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６个水囊引产４～５个月龄人胚肢体肌组织，采用０．１％Ⅱ型胶原酶和０．１２５％胰蛋白酶二步消化法，分离肌卫星细胞，在含胎肌条件培养液（ＨＦＭＥ）的培养基中，差速贴壁及克隆纯化，电镜及Ｄｅｓｍｉｎ免疫细胞化学鉴定纯度后，Ｒｕｂｂｅｒｐｏｌｉｃｅ刮除收集，按１．６×１０６个细胞／ｋｇ体重，间隔０．５ｃｍ点均匀移植于恒河猴肱二头肌组织中。结果表明，该方法培养的成肌细胞纯度达９６％以上，移植后血清肌浆酶ＬＤＨ及ＣＰＫ与移植前相近（Ｐ＞０．０５），肌组织活检未见明显坏死，表明成肌细胞移植无宿主肌纤维损伤作用；移植后１周ＣＤ４／ＣＤ８比值大于２，补体Ｃ４短暂下降，但Ｔａｃ各值未超过移植前，２周后ＣＤ４／ＣＤ８比值恢复到正常水平，表明恒河猴对移植人的成肌细胞有排异反应，但可以耐受。移植后１周肌组织中可以检出Ｄｅｓｍｉｎ（＋）成肌细胞生长及移植后２周的ＡＯ（＋）的嗜碱性再生肌纤维，此后早期呈ＨＬＡ－ＤＲ（＋）的ＣＤ３（＋）、ＣＤ８（＋）细胞几尽消退。
Six water sacs induced 4 to 5 months old human embryonic limb muscle tissue using 0.1% type Ⅱ collagenase and 0.125% trypsin two-step digestion, isolated muscle satellite cells in fetal culture fluid containing conditions (HFME), differential adherence and cloning and purification, electron microscopy and Desmin immunocytochemistry to identify the purity, Rubberpolice scrape collection, according to 1.6 × 106 cells / kg body weight, evenly spaced 0.5cm point transplant In Rhesus monkey biceps tissue. The results showed that the purity of myoblasts cultured by this method was more than 96%. The LDH and CPK of serum sarcoplasm after transplantation were similar to those before transplantation (P> 0.05), and no significant necrosis was found in muscle biopsy, indicating that myoblast transplantation The ratio of CD4 / CD8 was higher than 2 at 1 week after transplantation, and the expression of C4 was transiently decreased. However, the Tac values did not exceed the values before 4 weeks after transplantation. The ratio of CD4 / CD8 returned to normal level after 2 weeks of transplantation, Myoblasts have rejection, but can be tolerated. At 1 week after transplantation, the basophilic regenerated myofibers of Desmin (+) myoblasts and AO (+) 2 weeks after transplantation were detected, and the CD3 (+) of HLA-DR CD8 (+) cells almost disappear.