ACh 一般储存于胆碱纯神经元的囊泡中,分子量小,缺乏抗原性,而且不稳定。要制得抗ACh 的抗体,首先必须建立起一套克服ACh 分子化学结构上的缺陷的免疫学方法。Geffard 等为了保持ACh 的结构同时赋予其抗原性,用化学方法给胆碱分子接上戊二酸酐(GA)及多肽(蛋白质),经此修饰后的胆碱-戊二酸酐-蛋白质与ACh 极相似,而且具有抗原性;继而免疫动物即可制得针对ACh 的抗体。他们又对组 ACh is normally stored in vesicles of pure cholinergic neurons with low molecular weight, lack of antigenicity, and is unstable. To produce anti-ACh antibodies, we must first establish a set of immunological methods to overcome the chemical structural defects of ACh molecules. Geffard and so in order to maintain the structure of ACh while giving its antigenicity, chemical methods to connect choline molecules glutaric anhydride (GA) and peptides (proteins), the modified choline - glutaric anhydride - protein and ACh pole Similar, but also has antigenicity; then the animals can be immunized against ACh antibodies. They are right again