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采用生物素标记的寡核苷酸探针,应用改良的原位杂交方法,检测了鼻咽癌及淋疤结转移癌标本石蜡包埋组织中的EB病毒mRNA。结果显示,EB病毒阳性反应清晰,无非特异性背景。结果表明,本实验中所应用的探针及改良的原位杂交方法可以迅速准确地检测组织内EB病毒mRNA的存在。
Biotinylated oligonucleotide probes were used to detect Epstein-Barr virus mRNA in paraffin-embedded tissues of nasopharyngeal carcinoma and lymph node metastatic carcinoma specimens using a modified in situ hybridization method. The results showed that EB virus positive reaction was clear, non-specific background. The results showed that the probe used in this experiment and modified in situ hybridization method can quickly and accurately detect the presence of EB virus mRNA in the tissue.