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目的探讨T-钙黏蛋白对B16F10黑素瘤细胞周期的影响。方法应用脂质体LipofectamineTM2000将构建的pEGFP-N1-T-钙黏蛋白以及pEGFP-N1空载体转染至B16F10细胞,OPTI-MEM培养基体外培养6 h后RPMI-1640继续培养24 h,应用碘化丙啶标记,流式细胞仪检测T-钙黏蛋白对B16F10黑素瘤细胞周期的影响。结果转染T-钙黏蛋白组G0/G1期和G2/M期细胞比率分别为(60.917±1.897)%、(8.513±0.651)%,明显高于未转染组的(53.563±1.856)%、(2.627±0.434)%和pEGFP-N1空载体组的(53.880±2.164)%、(2.770±0.466)%,差异有显著性(P<0.05);转染T-钙黏蛋白组S期细胞比率为(30.570±1.368)%,低于未转染组的(43.810±2.003)%及pEGFP-N1空载体组的(43.350±2.629)%,差异有统计学意义(P<0.05)。未转染组与转染pEGFP-N1空载体组各期差异均无统计学意义(P>0.05)。结论 T-钙黏蛋白使B16F10细胞G0/G1期和G2/M期比率增高,S期比率降低。
Objective To investigate the effect of T-cadherin on the cell cycle of B16F10 melanoma cells. METHODS: The constructed pEGFP-N1-T-cadherin and pEGFP-N1 vector were transfected into B16F10 cells by LipofectamineTM2000. After cultured in OPTI-MEM medium for 6 h, RPMI-1640 cells were cultured for 24 h. The effect of T-cadherin on the cell cycle of B16F10 melanoma cells was examined by using propidium iodide and flow cytometry. Results The percentage of cells in G0 / G1 and G2 / M phases transfected with T-cadherin was (60.917 ± 1.897)% and (8.513 ± 0.651)%, respectively, which was significantly higher than that of untransfected cells (53.563 ± 1.856% (2.627 ± 0.434)% and (53.880 ± 2.164)% and (2.770 ± 0.466)% respectively in pEGFP-N1 empty vector group (P <0.05) (30.570 ± 1.368)%, lower than that of untransfected group (43.810 ± 2.003)% and pEGFP-N1 empty vector group (43.350 ± 2.629)%, the difference was statistically significant (P <0.05). There was no significant difference between the untransfected group and the blank vector transfected with pEGFP-N1 (P> 0.05). Conclusions T-cadherin increased the ratio of G0 / G1 phase and G2 / M phase in B16F10 cells, but decreased in S phase.