为扩大生产，采用５００立升发酵罐培养无细胞百日咳菌苗，发现随着培养时间的延续，细胞浓度增高，培养液的ｐＨ值上升，ＰＴ、ＦＨＡ活性、血凝效价逐渐增加、Ｏ２溶压下降、ＣＯ２溶压上升。ｐＨ值达８２时，ＰＴ活性最高为３００ＥＵ／ｍｌ，较现用扁瓶培养方法高５倍。ｐＨ值继续上升时，ＰＴ活性开始下降。ＦＨＡ活性及血凝效价具有相似的变化。通过测定培养液ｐＨ值以确定收获时间，可获得富含ＰＴ、ＦＨＡ、且活性均保持较高水平的培养液 In order to expand production, acellular pertussis vaccine was cultured in a 500-liter fermentor. It was found that as the incubation time prolonged, the cell concentration increased, the pH value of the culture broth increased, the activity of PT and FHA, the titer of blood coagulation gradually increased, Pressure drop, CO2 pressure rise. The highest PT activity was 300EU / ml at pH 82, which was 5 times higher than that of flat flask culture. As pH continues to rise, PT activity begins to decrease. FHA activity and blood coagulation titer with similar changes. By measuring the pH value of the culture medium to determine the harvest time, it is possible to obtain a culture medium rich in PT, FHA, and having a high activity level