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50年代后期,由于分子遗传学的迅速发展,以及细胞培养法的广泛应用,大大促进了肿瘤病毒病因学的研究,尤其是在RNA肿瘤病毒的致癌作用方面积累了大量的资料。放线菌素D能抑制由DNA模板(Template)合成RNA。后来又发现它也能抑制Rous肉瘤病毒(RSV)的复制。但RSV只含有RNA,不含DNA,为什么也会被放线菌素D所抑制呢?1964年,Temin根据这一事实提出了著名的“前病毒”(provirus)假说。他设想RSV的基因组(genome)可能先录写
In the late 1950s, due to the rapid development of molecular genetics and the widespread use of cell culture methods, the etiology of tumor viruses has been greatly promoted. In particular, a large amount of data have been accumulated in the carcinogenesis of RNA tumor viruses. Actinomycin D inhibits the synthesis of RNA from DNA templates. It was later found that it also inhibited the replication of Rous sarcoma virus (RSV). But RSV contained only RNA, DNA-free, and why was it suppressed by actinomycin? In 1964 Temin proposed the famous “provirus” hypothesis based on this fact. He envisaged that the RSV genome might be first recorded