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目的采用HPLC法测定腺毛千斤拔、宽叶千斤拔的根茎及大叶千斤拔、蔓性千斤拔不同产地的根中β-谷甾醇的含量。方法色谱柱为Kromasil C18(250mm×4.6mm,5μm),流动相为甲醇,流速1.0mL·min-1,柱温25℃。结果β-谷甾醇0.65～3.25μg与峰面积呈良好的线性关系,平均回收率为99.40%,RSD=0.87%(n=9)。腺毛千斤拔、宽叶千斤拔的根和茎中β-谷甾醇含量均较高;湖北宜昌的蔓性千斤拔及成都华西植物园的大叶千斤拔的根中β-谷甾醇含量最高,其他产地含量较低;大叶千斤拔根中β-谷甾醇的含量较蔓性千斤拔高。结论所用方法简便、快速、准确,可控制千斤拔药材的质量。
OBJECTIVE To determine the content of β-sitosterol in the root of Tricholoma giganteus, Radix angustifolium, and Radix maxima in different habitats by HPLC. Methods The column was Kromasil C18 (250mm × 4.6mm, 5μm). The mobile phase was methanol and the flow rate was 1.0mL · min-1. The column temperature was 25 ℃. Results There was a good linear relationship between β-sitosterol and peak area with the average recoveries of 99.40% and RSD of 0.87% (n = 9). The content of β-sitosterol in the root and stem of the broad-leaf jackfruit was higher. The contents of β-sitosterol in the root of the jackfruit in the Yichang of Hubei and the root of the jackfruit in the Huaxi Botanical Garden of Chengdu were the highest, while the others Lower content of origin; large leaves jackfruit root in β-sitosterol content than clogged jacking high. Conclusions The method used is simple, rapid and accurate, and can control the quality of the unplug medicine.