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目的：动态观察吗啡对小鼠免疫功能的影响。方法：制作吗啡急性作用和吗啡依赖小鼠模型，以盐水及空白组为对照。分离单个核细胞，用间接免疫荧光检测Ｔ细胞亚群，加ＣｏｎＡ、ＬＰＳ进行２４小时培养，检测上清中ＩＬ－６、ＩＬ－２、ＮＯ水平。结果：注射吗啡（８０ｍｇ／ｋｇ／ｄ）７天后出现淋巴器官重量的减轻，Ｔ细胞亚群的改变，细胞因子ＩＬ－６显著升高，ＩＬ－２显著降低，ＮＯ水平在注射吗啡１、３、５天时显著升高。结论：吗啡可抑制小鼠的免疫功能，ＴＨ１细胞和ＴＨ２细胞的功能失衡可能是吗啡影响免疫系统的一个中介因素。
Objective: To dynamically observe the effect of morphine on immune function in mice. Methods: Acute morphine and morphine-dependent mouse models were made, with saline and blank control as controls. The mononuclear cells were isolated, T lymphocyte subsets were detected by indirect immunofluorescence, and cultured with ConA and LPS for 24 hours. The levels of IL-6, IL-2 and NO in supernatants were detected. RESULTS: After 7 days of morphine injection (80 mg / kg / d), the weights of lymphoid organs and the changes of T lymphocyte subsets were significantly increased. The levels of cytokines IL-6 and IL-2 were significantly decreased. , 5 days increased significantly. CONCLUSION: Morphine can inhibit the immune function in mice. The imbalance between TH1 cells and TH2 cells may be an intermediary factor that morphine affects the immune system.