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目的:探讨莪术瓜蒌汤含药血清对体外培养的PGLH7细胞增生、细胞周期和凋亡的影响。方法:不同剂量莪术瓜蒌汤含药血清处理体外培养的PGLH7细胞后,采用MTT法观察血清对细胞增殖的作用;PI染色、流式细胞仪进行细胞周期时相分析;AnnexinV-FITC/PI双染色、流式细胞仪检测细胞凋亡率。结果:高剂量莪术瓜蒌汤血清组能明显抑制PGLH7细胞增生;流式细胞仪分析表明:中、高剂量莪术瓜蒌汤血清组处理PGLH7细胞24h、48h后,S期细胞百分率下降;细胞凋亡率上升,呈剂量依赖性。结论:本实验提示莪术瓜蒌汤含药血清可能是通过阻滞细胞周期和诱导细胞凋亡而抑制肺癌细胞的增殖。
OBJECTIVE: To investigate the effects of the serum containing Zhizhu Guaying decoction on proliferation, cell cycle and apoptosis of PGLH7 cells cultured in vitro. METHODS: After treated with different doses of Yuzhuo Guaying Decoction serum in vitro, the effect of serum on cell proliferation was observed by MTT assay; PI staining and flow cytometry were used to analyze the cell cycle; AnnexinV-FITC/PI double Staining and flow cytometry were used to detect the apoptosis rate. RESULTS: High-dose Zhizhuo Guayu decoction serum group could significantly inhibit the proliferation of PGLH7 cells. Flow cytometry analysis showed that the percentage of cells in S phase decreased after treatment of PGLH7 cells with medium and high doses of Qizhuo Guaying Decoction for 24 h and 48 h; The death rate increased in a dose-dependent manner. Conclusion: This experiment suggests that the serum contained in Zhizhuo Guaying Decoction may inhibit the proliferation of lung cancer cells by blocking the cell cycle and inducing apoptosis.