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目的观察阿托伐他汀和卡托普利干预对连续周期性波动的高静水压下大鼠肾小球系膜细胞(RMC)细胞外基质(ECM)的影响。方法RMC分别在生理压力(40mmHg,PP)、中压(60mmHg,MP)、高压(80mmHg,HP)环境下,不同药物(阿托伐他汀、卡托普利)中培养1、3、5、7d。采用Western Blotting法测定细胞裂解液中胶原Ⅰ和Ⅳ、纤维连结生长因子(FN)的含量。结果与PP组第1天相比,PP组第3、5、7天胶原Ⅰ/Ⅳ、FN浓度无明显变化;MP和HP组从第1天开始可见胶原Ⅰ/Ⅳ、FN水平呈时间依赖性升高,7d达到最高。阿托伐他汀和卡托普利均能抑制MP、HP组胶原Ⅰ/Ⅳ和FN水平上升,胶原Ⅰ在1d时,阿托伐他汀吸光度(A)值为1.06±0.12,1.16±0.14vs0.78±0.05;卡托普利A值为0.96±0.09,1.02±0.13vs0.71±0.06(P<0.05)。二者合用可使胶原Ⅰ/Ⅳ和FN水平进一步下降。结论周期性波动的高静水压可使ECM积聚。阿托伐他汀和卡托普利能减轻高静水压引起的ECM积聚。二者合用存在协同作用。
Objective To observe the effects of atorvastatin and captopril on the extracellular matrix (ECM) of rat mesangial cells (RMC) under continuous hydrostatic pressure (CVP). Methods RMCs were cultured in different drugs (atorvastatin, captopril) under physiological pressure (40mmHg, PP), medium pressure (60mmHg, MP) and high pressure (80mmHg, HP) 7d. Western Blotting method was used to determine the content of collagen Ⅰ and Ⅳ and fibronectin (FN) in the cell lysate. Results Compared with day 1 of PP group, there was no significant change in collagen Ⅰ, Ⅳ and FN concentrations on days 3, 5, and 7 in PP group. The levels of collagen Ⅰ / Ⅳ and FN in group MP and HP were time-dependent Sexual increase, reached the highest 7d. Both atorvastatin and captopril inhibited the levels of collagen Ⅰ / Ⅳ and FN in MP and HP groups. The absorbency (A) of atorvastatin at 1.0d was 1.06 ± 0.12 and 1.16 ± 0.14vs0 respectively. 78 ± 0.05; Captopril A values were 0.96 ± 0.09, 1.02 ± 0.13 vs 0.71 ± 0.06 (P <0.05). The combination of the two can further reduce collagen Ⅰ / Ⅳ and FN levels. Conclusions The cyclically fluctuating hydrostatic pressure can cause ECM accumulation. Atorvastatin and captopril reduce ECM accumulation caused by high hydrostatic pressure. There is synergy between the two.