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金黄色葡萄球菌引起人类多种严重感染,多重耐药的金葡菌导致院内感染逐年增加,流行病学调查需要将金葡菌分型.常用的金葡菌分型方法有:噬菌体分型、荚膜多糖血清型及酶分型、核酸分型和质粒分型.作者等利用SmaI消化金葡菌染色体DNA然后进行脉冲场凝胶电泳,依酶切图形对流行病学无关的两个来源的69株金葡菌分型(36株来自法国.33株来自德国的囊性纤维化病人的4周夏令营地),并与噬菌体、荚膜和酶分型法比较.
Staphylococcus aureus cause a variety of serious human infections, multi-drug-resistant Staphylococcus aureus led to increased year-to-year nosocomial infections, epidemiological investigations need to Staphylococcus aureus typing commonly used Staphylococcus aureus typing methods are: phage typing, Capsular polysaccharide serotypes and enzyme typing, nucleic acid typing and plasmid typing. The authors use SmaI digestion of S. aureus chromosomal DNA and then pulsed-field gel electrophoresis, according to the enzyme digestion patterns have nothing to do with the two epidemiological sources Sixty-nine Staphylococcus aureus strains were screened (36 at a four-week summer camp from France, 33 from cystic fibrosis patients in Germany) and compared with phage, capsular and enzymatic typing.