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本文比较了从甲醛固定的石蜡切片中提取DNA的几种方法,以用非同位索性PCR实验的效果。结果显示:用蛋白酶K继以煮沸(不用酚/氯仿抽提),效果最好,而用蛋白酶K继的/氯酚仿提取,其效果并不比单纯煮沸好。从甲醛固定石蜡切片中提取DNA用于非同位素PCR法中的方法学比较
This article compares several methods for extracting DNA from formalin-fixed paraffin sections to test the effect of non-isotopic PCR. The results showed that the best effect was obtained with proteinase K followed by boiling without phenol / chloroform extraction. However, the effect was not better than that of simple boiling with proteinase K / chloroform. Methodological comparison of DNA extraction from paraformaldehyde fixed paraffin sections for nonisotopic PCR