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目的探讨胃癌组织H pylori cagA感染对环氧合酶2(COX2)表达的影响。方法采用原位聚合酶链反应(PCR)及免疫组织化学技术检测60例慢性浅表性胃炎6、0例慢性萎缩性胃炎、64例肠上皮化生6、4例不典型增生、64例胃癌组织中cagA基因及COX2蛋白表达情况。结果COX2在胃癌、异型增生的阳性表达率升高,分别为70.3%、64.1%与肠上皮化生、萎缩性胃炎、浅表性胃炎相比有显著性差异(P<0.05)。在H pyloricagA阳性胃癌组、异型增生组COX2表达率明显高于H pyloricagA阴性组,两组之间有显著性差异(P<0.05);COX2阳性表达与患者性别、年龄、肿瘤大小无关,与患者浸润深度、淋巴转移、H pyloricagA感染有关。结论H pylori可通过诱导COX2表达参与胃癌病变,COX2参与胃癌的发生发展,CagA是H pylori的重要致病因素,与COX2表达上调及胃癌的发生密切相关。
Objective To investigate the effect of H pylori cagA infection on the expression of cyclooxygenase 2 (COX2) in gastric cancer. Methods 60 cases of chronic superficial gastritis, 60 cases of chronic atrophic gastritis, 64 cases of intestinal metaplasia, 6 cases of atypical hyperplasia, and 64 cases of gastric cancer were detected by in situ polymerase chain reaction (PCR) and immunohistochemistry. The expression of cagA gene and COX2 protein in tissues. Results The positive expression rate of COX2 in gastric cancer and dysplasia increased, 70.3% and 64.1%, respectively, compared with intestinal metaplasia, atrophic gastritis and superficial gastritis (P<0.05). The expression rate of COX2 was significantly higher in H.pyloricagA positive gastric cancer group and dysplasia group than in H.pyloricagA negative group. There was a significant difference between the two groups (P<0.05). The positive expression of COX2 was not related to the patient’s gender, age, and tumor size. Infiltration depth, lymphatic metastasis, H pylorigA infection. Conclusion H pylori can participate in the pathological changes of gastric cancer by inducing the expression of COX2. COX2 is involved in the occurrence and development of gastric cancer. CagA is an important pathogenic factor of H pylori, which is closely related to the up-regulation of COX2 expression and the occurrence of gastric cancer.