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神经白细胞素(NLK)相对分子量56KD,由去神经大鼠肌肉产生,多见于肌肉、脑、心脏和肾脏组织中。NLK 是脊髓和感觉神经元的神经因子,又是凝集素活化T细胞产生的淋巴因子。其功能是诱导人单核细胞分泌Ig,它的分子克隆已在猴COS 细胞表达,与其他多肽因子相似,10~(-9)～10~-(11)M浓度时具有活性。作者克隆猪肌肉的磷酸己糖异构酶(PHI,也称葡萄糖磷酸己糖异构酶GPI),此酶催化G-磷酸葡萄糖转变为6-磷酸果糖,是糖酵解途径限速酶之一。测GPI 的氨基酸顺序,竟与小鼠NLK 有90%的同源性。PHI 和NLK 的cDNA 克隆非常相似,且都有558个氨基酸残些,没有插入区或基因缺失。差异之处主要在保守区,可能是种族或脏器来源差异所致。以往认为PHI 分子不
Neutrophil (NLK) relative molecular weight of 56KD, produced by the muscle of the denervated rat, more common in muscle, brain, heart and kidney tissue. NLK is a neurological factor in the spinal cord and sensory neurons and is also a lymphokine produced by lectin-activated T cells. Its function is to induce human monocytes to secrete Ig, and its molecular clone has been expressed in monkey COS cells, similar to other polypeptide factors, and is active at the concentration of 10-9 (10 ~ - (11) M). The authors cloned porcine phosphohexose isomerase (PHI), also known as glucose phosphohexose isomerase GPI, which catalyzes the conversion of G-phosphoglucose to fructose-6-phosphate, one of the rate-limiting enzymes in the glycolysis pathway . The amino acid sequence of GPI was measured, which was actually 90% homologous to mouse NLK. The cDNA clones of PHI and NLK are very similar and both have 558 amino acid residues with no insertion region or gene deletion. The main difference in the conservative areas, may be caused by differences in ethnic origin or organ. In the past that PHI molecules do not