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目的 探讨饮食中添加牛磺酸对染矽尘大鼠肺组织诱导型一氧化氮合酶(iNOS)表达的作用。方法 采用气管暴露法建立矽肺动物模型,部分动物在饲料中添加牛磺酸(牛磺酸组) ;高效液相色谱法测定动物血浆中牛磺酸含量。链霉抗生物素蛋白-过氧化物酶法(SP法)结合组织芯片技术检测矽肺动物模型的肺组织iNOS的表达,用Image ProPlus图像分析系统对iNOS定量分析。结果 牛磺酸组大鼠各时间点血浆中牛磺酸含量高于对照组和染矽尘组,差异均有统计学意义(P <0 .0 5 )。染矽尘组大鼠支气管肺泡灌洗液(BALF)中总NOS活力、iNOS活力和肺组织iNOS阳性面积百分比都在染尘后第14天左右达高峰,分别比对照组增加1.84U/ml、1.12U/ml和5 .4 2 % ,差异均有统计学意义(P <0 .0 5 )。牛磺酸组与染矽尘组相比,BALF总NOS活力、iNOS活力和肺组织iNOS阳性面积百分比差异均无统计学意义(P >0 .0 5 )。结论 牛磺酸对矽尘所诱导的大鼠肺组织iNOS蛋白表达的增加未见明显影响
Objective To investigate the effect of adding taurine on the expression of inducible nitric oxide synthase (iNOS) in the lung tissue of rats exposed to silica dust. Methods The animal model of silicosis was established by tracheal exposure. Some animals were fed with taurine (taurine) and the content of taurine in plasma was determined by high performance liquid chromatography. Streptavidin-peroxidase method (SP method) combined with tissue microarray technology to detect the expression of iNOS in the lung tissue of the silicosis animal model, iNOS quantitative analysis with Image ProPlus image analysis system. Results The content of taurine in plasma of taurine group was higher than that of control group and silica dust group at each time point, the difference was statistically significant (P <0.05). The total NOS activity, iNOS activity and percentage of iNOS positive area in bronchoalveolar lavage fluid (BALF) of rats exposed to silica dust reached the peak on the fourteenth day after dust exposure, increasing by 1.84 U / ml than the control group, 1.12U / ml and5.42%, the differences were statistically significant (P <0. 05). There was no significant difference in total NOS activity, iNOS activity and iNOS positive area percentage of BALF between the taurine group and the silica dust group (P> 0.05). Conclusion Taurine has no obvious effect on the increase of iNOS protein expression induced by silica dust in rat lung