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目的：了解犬环杓后肌失神经后细胞增殖状态的变化规律，探讨反映肌肉不可逆改变的指标。方法：切断犬右喉返神经，对犬失神经环杓后肌进行如下观察：（１）ＨＥ染色测肌细胞的截面积；（２）Ｖａｎ－Ｇｉｅｓｏｎ染色测定胶原和肌细胞面积比；（３）ＣＡＳ２００图像分析系统ＤＮＡ定量测定方法统计Ｓ期细胞核比例。结果：环杓后肌肌细胞的截面积随时间延长逐渐减少；胶原纤维与肌细胞面积比随时间推移明显增高；３～４个月时Ｓ期细胞核所占百分比最高，以后减少。结论：犬环杓后肌失神经后４个月内保持高增殖水平，以后均降低，故神经修复术应力争早期进行。细胞增殖状态是反映肌萎缩是否可逆的潜在指标，有助于判断神经修复术的预后，为选择神经修复术的时机及方法提供临床依据
OBJECTIVE: To investigate the changes of proliferation status of canine posterior hypoglossal muscle in rats and to explore the indicators that reflect the irreversible changes of muscles. Methods: The right canine recurrent laryngeal nerve was cut off and the canine posterior cingulum was observed as follows: (1) HE staining was used to measure the cross-sectional area of myocytes; (2) the area ratio of collagen and myocyte was determined by Van-Gieson staining; (3) ) CAS200 Image Analysis System DNA Quantitation Assay S phase nuclear ratio. RESULTS: The cross-sectional area of posterior spinous muscle decreased gradually with the passage of time. The area ratio of collagen fibers to myocytes increased significantly with time. The percentage of S-phase nuclei was the highest at 3 to 4 months and then decreased. CONCLUSION: The canine posterior hypoglossal muscle maintains high proliferative rate within 4 months after the denervation of the posterior sclera of the canine and subsequently decreases. Therefore, the nerve repair should be strived for early. Cell proliferation status is a potential indicator of whether muscle atrophy is reversible, which can be helpful in judging the prognosis of nerve repair and providing a clinical basis for choosing the timing and method of nerve repair