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将纯化的基因重组HIV 1P2 4和融合蛋白P2 4 gp4 1包被微孔板 ,用ELISA分别检测正常人血清和HIV Ab国家参比品 (Panel)。rP2 4对 2 0份HIV Ab阳性Panel的检出率为 95 % (19/ 2 0 ) ,对 2 0份HIV Ab阳性Panel通过率为90 % (18/ 2 0 ) ,P2 4 gp4 1对 2 0份HIV Ab阳性Panel检出率为 90 % (18/ 2 0 ) ,对 2 0份HIV Ab阴性Panel的通过率为6 0 % (12 / 2 0 ) ;rP2 4和P2 4 gP4 1对正常人血清的检测结果均为阴性。结果表明研制的P2 4具有较高的敏感性和特异性 ,可作为组份抗原用于HIV抗体诊断试剂盒的生产
The purified recombinant HIV 1P2 4 and fusion protein P2 4 gp4 1 were coated on the microplate, and normal human serum and HIV Ab national standard were respectively detected by ELISA. The detection rate of rP2 4 against 20 HIV-1 positive Panels was 95% (19/2), 90% (20/2) over 20 HIV-1 positive Panels, P2 4 gp4 1 to 2 The detection rate of 0 HIV-positive panel was 90% (18/20), 60% (12/20) for 20 HIV-negative panels; rP2 4 and P2 4 gP4 1 were normal Human serum test results were negative. The results showed that the developed P2 4 has high sensitivity and specificity and can be used as component antigen in the production of HIV antibody diagnostic kit