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研究灭活SARS病毒的免疫原性。SARS病毒F6 9株经甲醛灭活后 ,加入氢氧化铝佐剂 ,以 3种剂量接种BALB/c小鼠 ,定时采血 ,测定特异性抗体的滴度及其中和活性 ,同时用化学发光酶联免疫法测定抗血清与SARS病毒结构蛋白的反应特异性及其相对强度。结果发现 ,小鼠接种疫苗 4d后 ,血清中可检测到IgM抗体 ,直至 2 6d后逐渐下降 ;IgG抗体在初次免疫 8d后出现 ,4 7d时达最高峰 ,6 3d后进入稳定期 ;不同剂量组的抗体滴度具有明显剂效关系 ,低剂量组和中剂量组滴度峰值为 1∶192 0 0 ,高剂量组滴度峰值为 1∶384 0 0。中和实验结果表明 ,小鼠所产生的抗体具有中和病毒活性 ,在 6 3d时 ,低剂量组和中剂量组血清的中和效价为 1∶12 80 ,高剂量组血清的中和效价为 1∶5 12 0。抗体分类结果表明 ,小鼠抗血清中含有针对多种SARS病毒结构蛋白的特异性抗体 ,其中 ,针对N蛋白、S4蛋白和S2蛋白的抗体水平相对较高 ,而抗M抗体、抗 3CL抗体的水平相对较低。上述结果说明 ,SARS病毒F6 9株经甲醛灭活后 ,各主要结构蛋白仍保持较强免疫原性 ;免疫小鼠后 ,可以诱导产生高滴度的特异性混合抗体 ,在体外可以保护敏感细胞不受SARS病毒攻击
To study the immunogenicity of inactivated SARS virus. SARS virus F6 strain 9 inactivated by formaldehyde, adding aluminum hydroxide adjuvant, BALB / c mice were inoculated with three doses, blood samples were collected regularly to measure the titer and neutralization activity of specific antibodies, The Specificity and Relative Intensity of Antiserum against Structural Protein of SARS Virus by Immunoassay. The results showed that IgM antibody could be detected in the sera of mice 4 days after vaccination, and gradually decreased after 26 days. The IgG antibody appeared after 8 days of initial immunization, reached the peak at 47 days and entered the stable phase at 6 days. The titer of the antibody titer in the group of low dose and medium dose was 1:192 0 0, and that of the high dose group was 1:384 0 0. The results of neutralization showed that the antibodies produced by mice had neutralizing virus activity. At 6 days, the neutralization titer of serum in low dose group and middle dose group was 1:1280. The neutralization effect of serum in high dose group The price is 1: 5 12 0. Antibody classification results showed that the mouse antisera contained specific antibodies to various SARS virus structural proteins, of which the antibody levels against the N protein, the S4 protein and the S2 protein were relatively high, while the anti-M antibody, anti-3CL antibody The level is relatively low. The above results indicate that the major structural proteins of SARS virus F6 strain 9 remain strong immunogenicity after being inactivated by formaldehyde. After being immunized, mice can be induced to produce high titers of specific mixed antibodies, which can protect sensitive cells in vitro Free from SARS virus attack