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非感光性莴苣（挂丝红品种）种子于２２℃下萌发率在８０％左右，而２８℃下不萌发。２２℃下外源亚精胺显著促进种子萌发，多胺生物合成抑制剂ＤＦＭＡ和ＤＣＨＡ明显抑制种子萌发，而ＤＦＭＯ无效。２２℃诱导种子萌发过程中内源腐胺和亚精胺含量显著高于２８℃下它们的含量。ＧＡ＿３处理能诱导２８℃下种子的萌发，单独外源亚精胺处理效果不大，而外源亚精胺对ＧＡ＿３诱导效应有促进作用。ＤＦ－ＭＡ和ＤＣＨＡ能抑制ＧＡ＿３诱导２８℃下种子的萌发，此抑制效应又能被外源亚精胺部分逆转。ＧＡ＿３处理也提高了２８℃下种子内源腐胺和亚精胺的含量。
Non-photosensitive lettuce (hanging silk varieties) seeds germination rate of about 80% at 22 ℃, and not germination at 28 ℃. Exogenous spermidine significantly promoted seed germination at 22 ° C. Polyamines biosynthesis inhibitors DFMA and DCHA significantly inhibited seed germination while DFMO was ineffective. The contents of endogenous putrescine and spermidine in 22 ℃ induced seed germination were significantly higher than those at 28 ℃. GA_3 treatment could induce the seed germination at 28 ℃, exogenous spermidine alone was not effective, while exogenous spermidine could promote GA_3 induction. DF-MA and DCHA could inhibit the seed germination induced by GA_3 at 28 ℃, and the inhibitory effect could be partially reversed by exogenous spermidine. GA_3 treatment also increased the content of endogenous putrescine and spermidine in seed at 28 ℃.