论文部分内容阅读
目的:研究新型细胞因子CKLFSF8对肿瘤细胞增殖及EGFR表达的作用。方法:首先利用RT-PCR了解CK-LFSF8基因在肿瘤细胞株BGC823和K562中的表达,然后在脂质体介导下将CKLFSF8基因转染这两种肿瘤细胞,倒置显微镜观察细胞生长,MTT法检测细胞增殖,免疫细胞化学法检测肿瘤细胞中EGFR的表达。结果:两种肿瘤细胞生长过程中均可测到CKLFSF8基因的表达。转染CKLFSF8后对BGC823和K562的增殖均有抑制作用,与空白对照组和空质粒组有统计学意义(P<0.05);CKLFSF8转染前后BGC823和K562细胞EGFR阳性率对比有统计学意义(P<0.05)。结论:CKLFSF8基因转染对肿瘤细胞的增殖和EGFR的表达有明显抑制作用,可望在肿瘤治疗中发挥重要作用。
Objective: To study the effect of novel cytokine CKLFSF8 on tumor cell proliferation and EGFR expression. Methods: The expression of CK-LFSF8 gene was detected by RT-PCR in tumor cell lines BGC823 and K562. Then the CKLFSF8 gene was transfected into both tumor cells by lipofectamine and the cell growth was observed by inverted microscope. MTT assay Cell proliferation was detected and EGFR expression in tumor cells was detected by immunocytochemistry. Results: The expression of CKLFSF8 gene was detected in both tumor cell growth. The transfection of CKLFSF8 could inhibit the proliferation of BGC823 and K562 cells, which was significantly different from that of blank control group and empty plasmid group (P <0.05). The positive rates of EGFR in BGC823 and K562 cells before and after CKLFSF8 transfection were statistically significant P <0.05). CONCLUSION: CKLFSF8 gene transfection can significantly inhibit tumor cell proliferation and EGFR expression and may play an important role in tumor therapy.