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【目的】探讨腹腔镜结直肠癌根治术对癌细胞脱落及腹膜组织、腹腔冲洗液中黏附分子（ICAM‐1、CD44v6、整合素β1）表达的影响。【方法】选择行结直肠癌根治术的结直肠癌患者65例，其中35例行腹腔镜结直肠癌根治术（腹腔镜组），30例行开腹结直肠癌根治术（开腹组）。分别于不同部位（切口处及远离切口处）采集两组患者的腹膜组织，并在肿瘤切除前和关腹前收集两组患者腹腔冲洗液，采用免疫组织化学法和双抗体夹心 ABC‐ELISA 法检测两组患者腹膜组织及腹腔冲洗液中黏附分子（ICAM‐1、CD44v6、整合素β1）的表达变化，并通过细胞学检查比较两种手术方法的腹腔冲洗液中癌细胞脱落情况。【结果】腹腔镜组、开腹组肿瘤切除前腹腔冲洗液中癌细胞检出率分别为8．6％（3／35）、13．3％（4／30），关腹前检出率分别为22．9％（8／35）、26．7％（8／30），两组关腹前后比较差异无统计学意义（ P ＞0．05）。两组肿瘤切除前和关腹前腹腔冲洗液、切开处和远离切口处腹膜组织中 ICAM‐1、CD44v6、整合素β1的表达水平比较，差异均无统计学意义（ P ＞0．05）。【结论】与传统开腹结直肠癌根治术比较，腹腔镜结直肠癌根治术腹腔内癌细胞脱落的风险并无提高，且对黏附分子 ICAM‐1、CD44v6及整合素β1的表达无影响。“,”Objective] To explore the effect of laparoscopy on cancer cells and the expressions of adhesion mole ‐cules (ICAM‐1 ,CD44v6 & integrin β1) in peritoneal tissue and abdominal rinse in patients with colorectal carcinoma (CC) .[Methods] A total of 65 CC cases undergoing radical resection at our hospital were analyzed .They were divid‐ed into two groups of LAP (laparoscopy ,n = 35) and OP (open surgery ,n = 30) .Peritoneal tissues were collected at incision and beyond .Also abdominal rinse was collected before tumor resection and abdomen closure .The expressions of adhesion molecules (ICAM‐1 ,CD44v6 & integrin beta 1) in peritoneal and abdominal cavities of two groups were detected by immunohistochemistry and double‐antibody sandwich ABC‐ELISA .And cancer cells in abdominal rinse were detected by peritoneal lavage cytology (PLC) for comparing two surgical methods .[Results] In LAP group , PLC was positive in 3 cases (8 .6% ) before tumor resection and 8 cases (22 .9% ) before abdominal closure .In OP group ,PLC was positive in 4 cases (13 .3% ) before tumor resection and 8 cases (26 .7% ) before abdominal closure . No inter‐group difference existed in PLC ( P > 0 .05) .The expressions of CD44v6 ,ICAM‐1 and integrin beta 1 in abdominal cavity (before tumor resection & abdomen closure ) were compared for LAP and OP groups .And there was no statistically significant difference ( P > 0 .05) .The expressions of CD44v6 ,ICAM‐1 and integrin beta 1 in peritoneal tissues (at incision and beyond) were compared between LAP and OP groups .And there was no statistical‐ly significant difference ( P > 0 .05) .[Conclusion] Compared with CC patients undergoing traditional open surgery , the risk of exfoliated cancer cells in abdominal cavity shows no increase .And there js no impact `upon the expressions of adhesion molecules .