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用基因工程重组人ＣＤ４分子免疫ＢＡＬＢ／ｃ小鼠，制备了一个分泌抗ＣＤ４ＭｃＡｂ的杂交瘤细胞株。该ＭｃＡｂ与ＣＤ４分子有强反应，其亲和力常数Ｋａｆｆ＝６．１２５×１０￣９Ｍ￣（－１）。竞争结合实验表明，它与ＡＴＣＣ克隆ＣＲＬ８００２（ＯＫＴ４）分泌的ＭｃＡｂ分别识别ＣＤ４分子中不同的表位，其腹水滴度较ＡＴＣＣ（ＯＫＴ４）ＭｃＡｂ腹水高约１０倍。
BALB / c mice were immunized with genetically engineered recombinant human CD4 molecules to produce a hybridoma cell line secreting anti-CD4McAb. The McAb and CD4 molecules have a strong reaction, the affinity constant Kaff = 6.125 × 10 ~ 9M ~ (-1). The competitive binding assay showed that McAb secreted by ATCC clone CRL8002 (OKT4) recognized different epitopes in CD4 molecule respectively, and the ascites titer was about 10 times higher than ascites of ATCC (OKT4) McAbs.