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【目的】观察田黄方不同组方对血脂及代谢产物的影响,揭示其治疗高脂血症的组方机制。【方法】取雄性SD大鼠30只,分为正常组、模型组、三七组、黄连组及田黄组。采用高脂乳剂灌胃法复制高脂血症大鼠模型并给予相应药物治疗,采集治疗4周后的血液,采用超高效液相色谱串联四级杆飞行时间质谱联用(UPLC/Q-TOF MS)技术建立大鼠血清的代谢物指纹图谱。应用主成分分析和偏最小二乘判别分析模型组和田黄组、三七组、黄连组之间的代谢物谱差异,并通过变量重要性投影(VIP)选取生物标志物,比较各组的标志物并找出差异。【结果】田黄方对饮食性高脂血症大鼠模型的干预作用主要体现在对脂肪代谢、氨基酸代谢、炎症和氧化应激的调节作用,而三七、黄连虽能一定程度改善模型组大鼠的代谢紊乱状态,但未能从根本上恢复。【结论】田黄方对高脂血症动物模型血脂及代谢产物的调节作用优于三七、黄连单用,其中三七以调节氨基酸代谢、能量代谢为主,黄连以调节炎症、氧化应激及能量代谢为主,田黄方对上述代谢紊乱均有调节作用。
【Objective】 To observe the effects of different prescriptions of Tianhuang Formula on blood lipids and metabolites, and to reveal its mechanism of treating hyperlipidemia. 【Methods】 Thirty male SD rats were divided into normal group, model group, panax notoginseng group, berberine group and Tianhuang group. The hyperlipidemic rat model was replicated by high-fat emulsion gavage and the corresponding drug treatment was given. The blood after 4 weeks of treatment was collected and purified by ultra-performance liquid chromatography tandem quadruple-time-of-flight mass spectrometry (UPLC / Q-TOF MS) technology to establish rat serum metabolite fingerprinting. Principal component analysis and partial least squares discriminant analysis were used to analyze the difference of metabolites between the model group and Tianhuang group, Sanqi group and Coptidis group, and biomarkers were selected by variable importance projection (VIP) And find the difference. 【Results】 Tianhuang prescription intervention in rats with diet-induced hyperlipidemia is mainly reflected in the regulation of fat metabolism, amino acid metabolism, inflammation and oxidative stress. While the effects of Huangqi Decoction can improve the model group to some extent Rat metabolic disorders, but failed to recover fundamentally. 【Conclusion】 Tianhuang decoction is superior to Panax notoginseng and coptis chinensis in regulation of blood lipid and metabolites of hyperlipemia animal model. Panax notoginseng regulates amino acid metabolism and energy metabolism, and Coptis chinensis regulates inflammation and oxidative stress And energy metabolism, Tianhuang Fang on the above metabolic disorders have a regulatory role.