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目的：观察膜磷脂成分改变对心肌肌浆网（ＳＲ）钙摄取功能的影响。方法：磷脂酶Ａ＿２或溶血磷脂分别与正常狗心肌ＳＲ温育，测定钙摄取量。结果：磷脂酶Ａ＿２可使ＳＲ摄钙量减少４８．８％（Ｐ＜０．０１），加入磷脂酰胆碱可部分逆转磷脂酶Ａ＿２造成的损伤。溶血磷脂酰胆碱或溶血磷脂酰丝氨酸显著抑制ＳＲ钙摄取。结论：磷脂酶Ａ＿２改变膜磷脂微环境，造成ＳＲ摄钙功能障碍可能是某些病理情况下，心肌细胞钙稳态失衡的原因之一。
OBJECTIVE: To observe the effects of changes in membrane phospholipid on calcium uptake in cardiac sarcoplasmic reticulum (SR). Methods: Phospholipase A 2 or lysophosphatidylcholine were respectively incubated with SR in normal dogs to measure calcium intake. Results: Phospholipase A 2 decreased the amount of SR uptake by 48.8% (P <0.01). The addition of phosphatidylcholine partly reversed the phosphorylation of phospholipase A 2. Lysophosphatidylcholine or lysophosphatidylserine significantly inhibited SR calcium uptake. CONCLUSION: Phospholipase A 2 can change membrane phospholipid microenvironment, resulting in SR-induced calcium dysfunction which may be one of the reasons for imbalance of calcium homeostasis in some pathological conditions.