论文部分内容阅读
目的 探讨检测尿液中端粒酶活性在膀胱癌诊断和监测复发中的意义。 方法 采用PCR ELISA法检测 4 6例膀胱癌患者尿液中的端粒酶活性 ,并观察其中 2 0例术后尿液端粒酶活性变化及与复发的关系。 结果 膀胱癌患者术前尿液端粒酶活性 0 .6 0± 0 .5 6 ,明显高于对照组 0 .18± 0 .0 8,P <0 .0 0 1,肿瘤切除后恢复正常 ,肿瘤复发前再次上升 ;端粒酶活性随肿瘤恶性程度的增加逐渐升高 ;术前端粒酶活性与早期复发无关。 结论 尿液端粒酶活性检测在膀胱癌诊断中有参考价值 ,与膀胱癌恶性程度相关 ,可能成为监测肿瘤复发的重要辅助手段。
Objective To investigate the significance of detecting telomerase activity in urine in diagnosis and recurrence of bladder cancer. Methods The telomerase activity in urine of 46 patients with bladder cancer was detected by PCR-ELISA. The changes of telomerase activity in urine and the relationship with recurrence were observed. Results The preoperative urinary telomerase activity of bladder cancer patients was significantly lower than that of the control group (0.60 ± 0.56, 0.18 ± 0.08, P <0.01), and returned to normal after tumor resection. Tumor recurrence increased again; telomerase activity increased with the increase of malignant degree of tumor; preoperative telomerase activity had nothing to do with early recurrence. Conclusion The detection of urinary telomerase activity has a reference value in the diagnosis of bladder cancer, which is related to the malignant degree of bladder cancer and may be an important adjunct to monitor tumor recurrence.