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抗寒剂CR-4,高油菜素内酯BR-120分别浸种和喷洒在水稻二叶期叶面上,经低温(2±1℃)暗胁迫2d,根干重、根长和茎叶干重,叶片的超氧物歧化酶(SOD),过氧化物酶(POX)活性和还原性谷胱甘肽(GSH)含量皆高于对照。抗性强的丽粳低温胁迫时清除活性氧的效果较好。回温恢复(25±1℃,3000lx、12h/d)1~5d、抗性不同的两个品种的SOD和POX活性、GSH和抗坏血酸(ASA)含量继续增加,丙二醛(MDA)含量都有下降。但抗性弱的秀子糯回温恢复时,清除活性氧的效果较好,POX活性和GSH含量增加较为显著。CR-4或BR-120处理在一定程度上提高了水稻幼苗活性氧清除系统的能力,增强了抗冷性。
The cold-resistant CR-4 and AA-BR-120 were soaked and sprayed on the leaves of the second leaf stage at low temperature (2 ± 1 ℃) for 2 days. The root dry weight, root length, The contents of superoxide dismutase (SOD), peroxidase (POX) and reducing glutathione (GSH) in the leaves and leaves of the two cultivars were higher than those of the control. Rejuvenation japonica japonica low temperature stress scavenging active oxygen better. The activity of SOD and POX, GSH and ascorbic acid (ASA) contents of two cultivars with different resistance were recovered after returning to normal temperature (25 ± 1 ℃, 3000lx, 12h / d) for 1 ~ 5d. MDA content There is decline. However, the weak resistance of the waxy temperature recovery, the removal of reactive oxygen species is better, POX activity and GSH content increased significantly. CR-4 or BR-120 treatment increased the ability of reactive oxygen species scavenging system of rice seedlings to a certain extent and enhanced the cold resistance.