A survey was made of the spore community of arbuscular mycorrhizal fungi (AMF) and root colonization by AMF in 16 plant species in Lhalu wetland on the outskirts of Lhasa city in Tibet. It was found that 13 of the 16 plant species investigated (81.5%) formed arbuscular mycorrhizal structures and dark septate endophytic fungi colonized the roots of most species. Total AMF colonization ranged from 0 to 82.6% in dicots and 0 to 54.5% in monocots. Both total AMF and arbuscular colonization were greater in dicots than that in monocots. A total of 48 taxa representing 7 genera of AMF were isolated and identified. Of these, 9 species belonged to Acaulospora, 2 to Appendicispora, 34 to Glomus, and 1 each to Pacispora, Paraglomus and Scutellospora. Spores of Glomus aggregatum, G. deserticola and G.etunicatum were most common and abundant in the spore survey. Spores of 8 to 26 AMF species were isolated from the rhizosphere soil of individual plant species. Spore densities in soil associated with the 16 plant species ranged from 20 to 475 per 20 g soil, with an average of (92.3±14.6). Species richness of AMF ranged from 6 to 12.7. There were no significant differences between dicots and monocots in AMF spore density or species richness. Future work directed towards understanding the response of the wetland plants to AMF may provide some insight into the role that these fungal symbionts may play in influencing plant diversity in this important urban wetland.rn