论文部分内容阅读
目的应用原子力显微镜(atomic force microscope,AFM)技术研究重组人内皮抑素(recombinant human endostatin,rhES)对内皮细胞的作用。方法采用MTT法检测不同浓度的rhES(0.05~2.4μg/ml)对人脐静脉内皮细胞ECV304增殖活力的影响;分别用0.8和2μg/ml的rhES处理ECV304细胞,应用AFM观察内皮细胞整体形貌的变化,SPI 3800 New DFM动力显微镜观察ECV304细胞表面局部形貌的变化。结果 rhES可明显抑制ECV304细胞增殖,且呈剂量效应(P<0.001);rhES可降低贴壁的ECV304细胞的厚度,且呈剂量依赖效应,使较光滑的细胞表面变粗糙,产生了一些微小的突起;经rhES处理的ECV304细胞表面结构呈现不规则的变化。结论 AFM技术具有样品制备简便和分辨率较高等优点,适合贴壁培养细胞的原位观察。
Objective To study the effect of recombinant human endostatin (rhES) on endothelial cells by atomic force microscope (AFM). Methods MTT assay was used to detect the effect of different concentrations of rhES (0.05-2.4μg / ml) on the proliferation of human umbilical vein endothelial cells (ECV304). The ECV304 cells were treated with rhES at 0.8 and 2μg / ml, respectively. Changes in the topography of ECV304 cells were observed using a SPI 3800 New DFM dynamic microscope. Results rhES inhibited the proliferation of ECV304 cells in a dose-dependent manner (P <0.001). RhES decreased the thickness of adherent ECV304 cells in a dose-dependent manner, roughened the smoother cell surface and produced some tiny The surface structure of ECV304 cells treated with rhES showed irregular changes. Conclusion AFM technology has the advantages of simple sample preparation and high resolution, suitable for in situ observation of adherent cells.