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目的 对影响青蒿丛生芽诱导因素进行基础性研究。方法 把常规的植物组织培养技术应用于调控青蒿中次生代谢产物青蒿素的生物合成代谢。结果 青蒿的基因型 ,激素和基本培养基对丛生芽的发生有显著影响 ,而光强在 10 0 0～ 6 0 0 0 lx和温度在 2 0℃～ 30℃对丛生芽的发生影响不大 ;在 5种基因型的青蒿中 ,0 2 5丛生芽的诱导率最高 ;诱导丛生芽的激素组合是 6 - BA2 .0 mg/ L 和 NAA0 .15 mg/ L;另外 ,离子在青蒿丛生芽的诱导和青蒿素的生物合成过程中起着非常重要的作用。结论 组织培养条件下 ,青蒿丛生芽的诱导及青蒿素的生物合成可以通过理化因子有效地进行调控。
Objective To study the basic factors affecting the inducing factors of clumps of Artemisia annua. Methods Conventional plant tissue culture techniques were applied to regulate the biosynthesis of artemisinin, a secondary metabolite in Artemisia annua. Results The genotypes, hormones and minimal medium of Artemisia annua had a significant effect on the occurrence of clustered shoots, but the light intensity at the temperature range of 1000-600 0x and temperature at 20 °C to 30 °C did not affect the occurrence of clustered shoots. Large; In the five genotypes of Artemisia annua, the 0.25 bud clusters had the highest induction rate; the clustered buds were 6-BA2.0 mg/L and NAA 0.15 mg/L, and the ions were blue. The induction of Artemisia Buds and the artemisinin biosynthesis play an important role. Conclusion Under the conditions of tissue culture, the induction of clumps of Artemisia annua and the biosynthesis of artemisinin can be effectively controlled by physical and chemical factors.