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目的:观察异常黑胆质成熟剂(ASMq)对抑郁症模型大鼠海马ERK信号通路的影响,探讨异常黑胆质成熟剂抗抑郁作用机制。方法:84只雄性SD大鼠,随机分为6组:正常对照组、抑郁症模型组、阳性对照组(氟西汀,3.5mg/kg)及异常黑胆质成熟剂低、中、高剂量组(浓度分别为1.5、3.0、6.0g/kg),每组14只。除正常对照组外,其它组采用慢性不可预见性温和应激(CUMS)诱导建立大鼠抑郁症模型,阳性对照组和异常黑胆质成熟剂各剂量组连续灌胃给药24天,1次/天。通过Open-field实验和糖水消耗实验来评估大鼠抑郁活动状态。末次给药24h后断头处死大鼠、迅速分离海马组织、冻存备用,蛋白印迹法(Western blot)检测大鼠海马p-ERK1/2,ERK1/2表达。结果:应激结束后与正常对照组相比,抑郁症模型组大鼠体重增加量降低、糖水消耗量减少,水平穿越格数、竖立次数均明显降低,处于抑郁状态;各组之间ERK1/2的表达没有显著性差异。与正常对照组相比,抑郁症模型组大鼠海马p-ERK1/2表达明显下降,差异有显著性。与抑郁症模型组相比,阳性对照组和异常黑胆质成熟剂中、高剂(浓度为3.0、6.0g/kg)量组大鼠体重增加量上升、糖水消耗量升高,水平穿越格数、竖立次数均明显增加;与抑郁症模型组相比,阳性对照组、异常黑胆质成熟剂中剂(浓度为3.0 g/kg)量组海马组织p-ERK1/2表达量明显升高,差异有显著性。结论:异常黑胆质成熟剂可能是通过调节ERK信号通路来发挥抗抑郁作用。
Objective: To investigate the effect of abnormal savda (ASMq) on ERK signal pathway in the hippocampus of depression model rats and to explore the antidepressant mechanism of abnormal savda mature agent. Methods: Eighty-four male Sprague-Dawley rats were randomly divided into 6 groups: normal control group, model group of depression, positive control group (fluoxetine, 3.5 mg / kg), and abnormal savda mature agent Group (concentration were 1.5,3.0,6.0 g / kg), 14 in each group. In addition to the normal control group, the rats in other groups were induced to establish depression model by chronic unpredictable mild stress (CUMS). The positive control group and the abnormal saffron maturation agent were administered intragastrically for 24 days and once /day. Depression activity in rats was assessed by Open-field experiments and sugar consumption experiments. The rats were sacrificed 24 hours after the last administration, the hippocampus was separated rapidly and stored for later. The expressions of p-ERK1 / 2 and ERK1 / 2 were detected by Western blot. Results: Compared with the normal control group, the rats in the depression model group had lower body weight gain, less water consumption, lower traverse grid number and erection frequency than those in the normal control group. The ERK1 / 2 expression was not significantly different. Compared with the normal control group, the expression of p-ERK1 / 2 in hippocampus of depression model group was significantly decreased, the difference was significant. Compared with the depression model group, in the positive control group and the abnormal saffron maturation agent, the body weight gain of the high dose group (3.0, 6.0 g / kg) increased, the consumption of sugar increased, The number of erection and the number of erection increased significantly. Compared with the depression model group, the expression of p-ERK1 / 2 in the hippocampus of the positive control group and the abnormal saffron maturation medium (concentration of 3.0 g / kg) , The difference was significant. Conclusion: Abnormal savda mature agent may exert antidepressant effect by regulating ERK signaling pathway.