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探讨黏附分子CD44V6的表达与乳腺癌浸润及腋淋巴结转移间的关系。应用链酶菌抗生物素蛋白 过氧化物酶染色法 (S P法)、抗原修复技术和RT PCR对正常乳腺组织、乳腺癌各 37例及部分 (16例 )腋淋巴结阳性组织进行CD44V6蛋白及mRNA的检测。结果显示 ,乳腺癌组织的CD44V6蛋白及mRNA阳性表达率均显著高于正常乳腺组织 (P <0 0 1) ,且与乳腺癌的TNM分期及腋淋巴结转移显著相关 (P <0 0 5 ) ;而与乳腺癌的组织学类型 ,患者年龄 ,肿块大小、部位 ,是否绝经等临床病理因素无关 ;两种方法的敏感性及在预测腋淋巴结转移率方面差异无显著性意义。研究表明 ,CD44V6蛋白及mRNA表达水平与乳腺癌的腋淋巴结转移密切相关 ,且随着乳腺癌TNM分期的提高 ,其阳性表达率明显增高 ;对乳腺癌组织进行CD44V6检测有助于预测乳腺癌病情进展及腋淋巴结的转移
To investigate the relationship between the expression of adhesion molecule CD44V6 and infiltration of breast cancer and axillary lymph node metastasis. Streptomyces avidin peroxidase staining (SP method), antigen retrieval techniques and RT PCR were used to detect the expression of CD44V6 protein and mRNA in 37 cases of normal breast tissues and 16 cases of axillary lymph node positive tissues The test. The results showed that the positive rates of CD44V6 protein and mRNA in breast cancer tissues were significantly higher than those in normal breast tissues (P <0.01), and were significantly correlated with TNM stage and axillary lymph node metastasis (P <0.05). However, it was not related to the histological type, age, mass size, location of menopause, and clinical pathological factors of breast cancer. The sensitivity of the two methods was not significant in predicting the axillary lymph node metastasis rate. Studies have shown that CD44V6 protein and mRNA expression levels are closely related to axillary lymph node metastasis of breast cancer, and with the TNM staging of breast cancer increased, the positive expression rate was significantly increased; CD44V6 detection of breast cancer tissue can help predict breast cancer Progress and axillary lymph node metastasis